Xinli Lin, Ph.D.

Associate Member, Functional Proteomics Laboratory

Director, Proteomics Core Facility

Adjunct Associate Professor, Department of Pathology, University of Oklahoma Health Sciences Center

My major interest is in the emerging research field of proteomics, with a short-term goal of expressing/refolding/purifying 50 to 100 “important” human proteins in their recombinant form. The approach is the use of a cost-effective method involving production of proteins as insoluble inclusion bodies, and then refold/purifying the proteins, using a “universal” procedure developed in this laboratory. More than 30 different proteins have been purified using the “universal” procedure. We have an ongoing NIH-supported project titled “Southeast Collaboratory for Structural Genomics,” which is a collaboratory effort between the University of Georgia, the University of Alabama, Birmingham (UAB), and other academic and industry centers, including OMRF, to develop high-throughput techniques for the determination of three-dimensional structures of proteins in genomic scale. Our lab uses the “universal” procedure to express/purify all genes from C. elegans, containing about 19,000 predicted protein-coding ORFs. Another NIH-supported project is to study new human aspartic proteases, which are important drug targets for human diseases such as Alzheimer’s disease. We also are involved in a gene therapy project, which is to develop new gene delivery systems for in vivo gene therapy. The gene therapy project is supported by OCAST.

Selected Publications:

Hong L, Koelsch G, Lin X, Wu S, Terzyan S, Ghosh A, Zhang XC and Tang J. Structure of memapsin 2 (beta-secretase) complexed with inhibitor: A template to design drugs for Alzheimer’s disease. Science 290:150-153, 2000.

Lin X, Koelsch G, Wu S, Downs D, Dashti A and Tang J. Human aspartic protease memapsin 2 cleaves the beta-secretase site of b-amyloid precursor protein. Proc Natl Aca Sci. (USA) 97(4):1456-1460, 2000.

Koelsch G, Tang J, Loy JA, Monod M, Jackson K, Foundling SI and Lin X. Enzymic characteristics of secreted aspartic proteases of Candida albicans. Biochimica et Biophysica Acta 1480:117-131, 2000.

Lin X. Construction of new retroviral producer cells from adenoviral and retroviral vectors. Gene Therapy 5:1251-1258, 1998.

Wang X, Lin X, Lowy JA, Tang J and Zhang XC. Crystal structure of the catalytic domain of human plasmin complexed with streptokinase. Science 281:1662-1665, 1998.

Lin, XL, Dashti A, Schinazi RF and Tang J. Intracellular diversion of glycoprotein gp160 of human immunodeficiency virus to lysosomes as a strategy of AIDS gene therapy. FASEB J 7:1070-1080, 1993.